Several tasks are only needed during the first night of a Clio run. These include:

Cold Stop Alignment

Focusing of Clio

The focus of the Clio camera is adjusted by changing the position of the AO WFS.
Once you have set this, the AO will keep Clio focused throughout the run.

Pupil Stop too lowPupil stop correctPupil Stop too high
Placement of pupil stop [f/20 big]: (left to right) too low, correct, too high. Use the balance in intensity of the four points at the corners to align.